A programmable $25 thermal cycler for PCR
نویسندگان
چکیده
منابع مشابه
PC-Based PCR Thermal Cycler
This paper presents a PC-based PCR thermal cycler with a host-local architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented host-local system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
متن کاملCost Reduction of PCR Thermal Cycler
This paper presents a PC-based thermal cycler with a local-host architecture, where a PC is employed as the host. The local system only executes the thermal cycling process and the PC is responsible for the management of PCR protocols with a convenient GUI. The presented local-host system greatly reduces the development time and the maintenance cost, providing an economical PCR thermal cycler.
متن کاملShortened PCR cycles in a conventional thermal cycler.
step. The presence of Vent DNA polymerase and PCR products other than the intended DNA linker, however, did not seem to interfere with subsequent reactions because ligations using both gel-purified and unpurified PCR products yielded a similar number of transformants. Eight transformants were tested, all of which contained the PCR product as an insert. The yield was considered to have high effi...
متن کاملA Rapid and Low-Cost PCR Thermal Cycler for Low Resource Settings
BACKGROUND Many modern molecular diagnostic assays targeting nucleic acids are typically confined to developed countries or to the national reference laboratories of developing-world countries. The ability to make technologies for the rapid diagnosis of infectious diseases broadly available in a portable, low-cost format would mark a revolutionary step forward in global health. Many molecular a...
متن کاملA battery-powered notebook thermal cycler for rapid multiplex real-time PCR analysis.
A compact, real-time PCR instrument was developed for rapid, multiplex analysis of nucleic acids in an inexpensive, portable format. The instrument consists of a notebook computer, two reaction modules with integrated optics for four-color fluorescence detection, batteries, and a battery-charging system. The instrument weighs 3.3 kg, measures 26 x 22 x 7.5 cm, and can run continuously on the in...
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ژورنال
عنوان ژورنال: Biochemistry and Molecular Biology Education
سال: 2003
ISSN: 1470-8175,1539-3429
DOI: 10.1002/bmb.2003.494031020190